Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Expression and Characterization of the Streptomyces coelicolor Serine/Threonine Protein Kinase PkaD
Hiroaki URABENarumi AOYAGIHiroshi OGAWARAKiyoto MOTOJIMA
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JOURNAL FREE ACCESS

2008 Volume 72 Issue 3 Pages 778-785

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Abstract

We identified and characterized the gene encoding a new eukaryotic-type protein kinase from Streptomyces coelicolor A3(2) M145. PkaD, consisting of 598 amino acid residues, contained the catalytic domain of eukaryotic protein kinases in the N-terminal region. A hydrophobicity plot indicated the presence of a putative transmembrane spanning sequence downstream of the catalytic domain, suggesting that PkaD is a transmembrane protein kinase. The recombinant PkaD was found to be phosphorylated at the threonine and tyrosine residues. In S. coelicolor A3(2), pkaD was transcribed as a monocistronic mRNA, and it was expressed constitutively throughout the life cycle. Disruption of chromosomal pkaD resulted in a significant loss of actinorhodin production. This result implies the involvement of pkaD in the regulation of secondary metabolism.

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© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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