A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase

Yeong-Deok KWON; Sang Yup LEE; Pil KIM

doi:10.1271/bbb.70831

Microbiology & Fermentation Technology Notes

A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase

Yeong-Deok KWON, Sang Yup LEE, Pil KIM

著者情報

キーワード: PEP carboxykinase, PEP carboxylase, intracellular ATP

ジャーナルフリー

2008 年 72 巻 4 号 p. 1138-1141

DOI https://doi.org/10.1271/bbb.70831

View "Advance Publication" version (April 7, 2008).

詳細

抄録

To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.

責任著者(Corresponding author)

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