Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Antisense RNA Inhibition of the β Subunit of the Dictyostelium discoideum Mitochondrial Processing Peptidase Induces the Expression of Mitochondrial Proteins
Koki NAGAYAMAShiori ITONOTakashi YOSHIDASei-ichi ISHIGUROHiroshi OCHIAITetsuo OHMACHI
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2008 Volume 72 Issue 7 Pages 1836-1846

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Abstract

We cloned and characterized a cDNA encoding the Dictyostelium discoideum β subunit of mitochondrial processing peptidase (Ddβ-MPP). Western blot analysis of the mitochondrial subfractions revealed that Ddβ-MPP is located in the mitochondrial matrix and membrane, whereas Ddα-MPP, another subunit of DdMPP, is located only in the matrix. Although expression of Ddβ-MPP mRNA is down-regulated during early development, the level of the Ddβ-MPP protein is constant throughout the Dictyostelium life cycle. In a transformant expressing the antisense RNA of the β-MPP gene, unexpectedly, the β-MPP protein increased about 1.8-fold relative to the wild type, and its mRNA increased 4.5-fold. Expression of other mitochondrial proteins, α-MPP and Cox IV, was also induced. These results suggest that antisense RNA inhibition of the β-MPP gene induces gene expression of mitochondrial proteins, presumably in a retrograde signaling manner. This is the pathway of the transfer of information from the mitochondria to the nucleus.

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© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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