Cloning and Functional Expression of an α-Galactosidase from Yersinia pestis biovar Microtus str. 91001

Yanan CAO; Huoqing HUANG; Kun MENG; Peilong YANG; Pengjun SHI; Yaru WANG; Huiying LUO; Zhifang ZHANG; Ningfeng WU; Bin YAO

doi:10.1271/bbb.80145

Biochemistry & Molecular Biology Notes

Cloning and Functional Expression of an α-Galactosidase from Yersinia pestis biovar Microtus str. 91001

Yanan CAO, Huoqing HUANG, Kun MENG, Peilong YANG, Pengjun SHI, Yaru WANG, Huiying LUO, Zhifang ZHANG, Ningfeng WU, Bin YAO

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Keywords: α-galactosidase, glycoside hydrolase, Yersinia pestis biovar Microtus str. 91001, characterization

JOURNAL FREE ACCESS

2008 Volume 72 Issue 8 Pages 2203-2205

DOI https://doi.org/10.1271/bbb.80145

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Abstract

A gene encoding a glycoside hydrolase (GH) family 36 α-galactosidase was cloned from Yersinia pestis biovar Microtus str. 91001 and expressed in Escherichia coli. The purified recombinant α-galactosidase (Aga-Y) was optimally active at 37 °C and pH 6.8. The features of temperature profile, thermoliability, kinetics, and amino acid composition indicated that Aga-Y had properties of a cold-adapted enzyme.

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