Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Production of Sophorolipid Biosurfactant by Pichia anomala
Jiraporn THANIYAVARNTanusta CHIANGUTHAIPolakit SANGVANICHNiran ROONGSAWANGKeji WASHIOMasaaki MORIKAWASuthep THANIYAVARN
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2008 Volume 72 Issue 8 Pages 2061-2068

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Abstract

Biosurfactant production by Pichia anomala PY1, a thermotorelant strain isolated from fermented food, was examined as grown in media containing various carbon and nitrogen sources. The optimal conditions for biosurfactant production included 4% soybean oil as carbon source at pH 5.5 at 30 °C for 7 d. Under these conditions, the surface tension of the medium decreased to 28 mN/m with oil displacement measured at 69.43 cm2. Comparative studies of biosurfactant production in media containing glucose or soybean oil were performed. The biosurfactants obtained were isolated and purified by chromatographic methods. The molecular weights of samples were further investigated by mass spectrometry. In medium containing glucose, biosurfactants of molecular weights of 675, 691, and 707 were obtained, while those isolated from medium containing soybean oil were of molecular weights of 658, 675, and 691. These results reveal that sophorolipid compounds containing fatty acids of C20 and C18:1 were produced from both media.

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© 2008 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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