Conversion of 5-Hydroxytryptophan into Serotonin by Tryptophan Decarboxylase in Plants, Escherichia coli, and Yeast

Munyoung PARK; Kiyoon KANG; Sangkyu PARK; Kyoungwhan BACK

doi:10.1271/bbb.80220

Biochemistry & Molecular Biology Notes

Conversion of 5-Hydroxytryptophan into Serotonin by Tryptophan Decarboxylase in Plants, Escherichia coli, and Yeast

Munyoung PARK, Kiyoon KANG, Sangkyu PARK, Kyoungwhan BACK

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Keywords: tryptophan decarboxylase, 5-hydroxytryptophan decarboxylase, 5-hydroxytryptophan, serotonin

JOURNAL FREE ACCESS

2008 Volume 72 Issue 9 Pages 2456-2458

DOI https://doi.org/10.1271/bbb.80220

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Abstract

The L-tryptophan decarboxylase (TDC) gene of rice was heterologously expressed in various organisms. Transgenic rice overexpressing TDC showed accumulation of serotonin upon 5-hydroxytryptophan treatment, which was consistent with the in vitro 5-hydroxytryptophan decarboxylase enzyme activity of purified recombinant rice TDC in a pyridoxal phosphate-dependent manner. Recombinant yeast harboring TDC produced serotonin at the expense of the endogenous 5-hydroxytryptophan levels.

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