Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)

Andian Ari ANGGRAENI; Makiko SAKKA; Tetsuya KIMURA; Khanok RATANAKHAOKCHAI; Motomitsu KITAOKA; Kazuo SAKKA

doi:10.1271/bbb.80242

Microbiology & Fermentation Technology Notes

Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)

Andian Ari ANGGRAENI, Makiko SAKKA, Tetsuya KIMURA, Khanok RATANAKHAOKCHAI, Motomitsu KITAOKA, Kazuo SAKKA

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Keywords: Bacillus halodurans, α-galactosidase, melibiase, glycoside hydrolase, raffinose

JOURNAL FREE ACCESS

2008 Volume 72 Issue 9 Pages 2459-2462

DOI https://doi.org/10.1271/bbb.80242

View "Advance Publication" version (September 7, 2008).

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Abstract

A family-4 α-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD⁺, some divalent cations such as Mn²⁺, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.

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