Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Detection of 5-Lipoxygenase Activity in the Liverwort Marchantia polymorpha L.
Hirosuke KANAMOTOMiho TAKEMURAKanji OHYAMA
Author information
JOURNAL FREE ACCESS

2009 Volume 73 Issue 11 Pages 2549-2551

Details
Abstract

We detected 5-LOX (arachidonate 5-lipoxygenase) in the homogenate of Marchantia polymorpha by spectrophotometry and mass spectrometry. LC/MS/MS analysis indicated that the liverwort 5-LOX produced 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) with arachidonic acid as a substrate. The 5-LOX activity showed a Ca2+ response, as demonstrated for human 5-LOX. These findings suggest that the liverwort utilizes an arachidonate cascade in a defense signal response.

Content from these authors

This article cannot obtain the latest cited-by information.

© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top