Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communication
Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
Shinya NAKAMURAAkihide NAKAOMakoto KAWAMUKAITetsuya KIMURASumie ISHIGUROTsuyoshi NAKAGAWA
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2009 Volume 73 Issue 11 Pages 2556-2559

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Abstract

We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were β-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).

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© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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