Purification of Inactive Precursor of Carboxypeptidase Y Using Selective Cleavage Method Coupled with Molecular Display

Abstract

A novel purification system for inactive precursor proteins without conventional time-consuming purification steps was established. We purified an inactive precursor protein, procarboxypeptidase Y (proCPY), which was displayed on the cell surface of yeast, due to difficulty of purifying it by a system in which it is produced in cells.