Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Transformation of Arabidopsis with Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene
Aftab AHMAD, Izumi KAJI, Yukiko MURAKAMI, Nana FUNATO, Takeshi OGAWA, Masanori SHIMIZU, Yasuo NIWA, Hirokazu KOBAYASHI
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2009 年 73 巻 4 号 p. 936-938

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Most genetically modified plants are made with antibiotic-resistant genes and promoters derived from plant pathogens. To promote public acceptance, we have adapted a selectable marker and promoters all of which were derived from Arabidopsis thaliana to transformation. We have employed the gene for acetolactate synthase with its own promoter as a selectable marker, and the actin 2 gene promoter to strongly express an objective gene.
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© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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