Comparative Enzymatic Analysis of Azoreductases from Bacillus sp. B29

Toshihiko OOI; Takeshi SHIBATA; Ken’ichiro MATSUMOTO; Shinichi KINOSHITA; Seiichi TAGUCHI

doi:10.1271/bbb.80872

Biochemistry & Molecular Biology Notes

Comparative Enzymatic Analysis of Azoreductases from Bacillus sp. B29

Toshihiko OOI, Takeshi SHIBATA, Ken’ichiro MATSUMOTO, Shinichi KINOSHITA, Seiichi TAGUCHI

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Keywords: azo dyes, azoreductase, substrate specificity, flavoprotein, Bacillus sp. B29

JOURNAL FREE ACCESS

2009 Volume 73 Issue 5 Pages 1209-1211

DOI https://doi.org/10.1271/bbb.80872

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Abstract

We cloned and expressed two genes encoding azoreductase homologes, AzrB and AzrC, from Bacillus sp. B29. Purified recombinant AzrB and AzrC were homodimers with 23 kDa identical subunits, and were flavoproteins. NADH was preferred as electron donors for both azoreductases. The azoreductases showed optimal activities at 70 °C (AzrB) and 55 °C (AzrC), and retained activities up to 55 °C (AzrB) and 50 °C (AzrC) after incubation for 1 h. Other enzymatic properties, including the substrate specificities of both azoreductases, were also investigated.

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