Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Analytical Chemistry Regular Paper
Sensitive Quantification of Bulleyaconitine A in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry
Xin XIONGSuodi ZHAIZhongqiang YAO
Author information
JOURNALS FREE ACCESS

2009 Volume 73 Issue 7 Pages 1572-1577

Details
Abstract

A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1×50 mm, 5 μm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition mz 644.2>584.3 for BLA and mz 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587–11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Next article
feedback
Top