Optical Detection of Specific Genes for Genetically Modified Soybean and Maize Using Multiplex PCR Coupled with Primer Extension on a Plastic Plate

Abstract

A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension primer-immobilized plate and the spots corresponding to the DNA sequences were visualized. This method is a rapid and simple way to detect GM soybean and GM maize optically.