Cloning and Characterization of the Promoter of the 9-cis-Epoxycarotenoid Dioxygenase Gene in Arachis hypogaea L.

Jianhua LIANG; Lixia YANG; Xiong CHEN; Ling LI; Dongliang GUO; Haihang LI; Biyu ZHANG

doi:10.1271/bbb.90133

Biochemistry & Molecular Biology Notes

Cloning and Characterization of the Promoter of the 9-cis-Epoxycarotenoid Dioxygenase Gene in Arachis hypogaea L.

Jianhua LIANG, Lixia YANG, Xiong CHEN, Ling LI, Dongliang GUO, Haihang LI, Biyu ZHANG

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Keywords: 9-cis-epoxycarotenoid dioxygenase, promoter, water stress, abscisic acid response element, Arachis hypogaea L.

JOURNAL FREE ACCESS

2009 Volume 73 Issue 9 Pages 2103-2106

DOI https://doi.org/10.1271/bbb.90133

View "Advance Publication" version (September 7, 2009).

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Abstract

We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. β-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by application of abscisic acid (ABA) and salicylic acid (SA) in transgenic Arabidopsis. Moreover, two novel ABRE-like (abscisic acid response element) elements were identified in the promoter region.

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