Suppressed-Priming PCR, a Novel Concept of DNA Quantification Based on PCR Kinetics

Abstract

The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurate and robust PCR-based quantification methods.