Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

Tomoyuki KONISHI; Yasumasa MIYAZAKI; Seiyei YAMAKAWA; Hiroaki IWAI; Shinobu SATOH; Tadashi ISHII

doi:10.1271/bbb.90619

Biochemistry & Molecular Biology Notes

Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

Tomoyuki KONISHI, Yasumasa MIYAZAKI, Seiyei YAMAKAWA, Hiroaki IWAI, Shinobu SATOH, Tadashi ISHII

著者情報

キーワード: arabinofuranose, cell-wall biosynthesis, reversibly glycosylated polypeptides, recombinant proteins, UDP-arabinopyranose mutase

ジャーナルフリー

2010 年 74 巻 1 号 p. 191-194

DOI https://doi.org/10.1271/bbb.90619

View "Advance Publication" version (January 7, 2010).

詳細

抄録

Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.

責任著者(Corresponding author)

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