Production of 4-Keto-<small>D</small>-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens

Osao ADACHI; Roque A. HOURS; Yoshihiko AKAKABE; Somboon TANASUPAWAT; Pattaraporn YUKPHAN; Emiko SHINAGAWA; Toshiharu YAKUSHI; Kazunobu MATSUSHITA

doi:10.1271/bbb.100698

Microbiology & Fermentation Technology Communications

Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens

Osao ADACHI, Roque A. HOURS, Yoshihiko AKAKABE, Somboon TANASUPAWAT, Pattaraporn YUKPHAN, Emiko SHINAGAWA, Toshiharu YAKUSHI, Kazunobu MATSUSHITA

Author information

Osao ADACHI
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
Roque A. HOURS
Research and Development Center for Industrial Fermentation (CINDEFI; UNLP, CONICET La Plata), School of Science, La Plata National University
Yoshihiko AKAKABE
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
Somboon TANASUPAWAT
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University
Pattaraporn YUKPHAN
BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
Emiko SHINAGAWA
Department of Chemical and Biological Engineering, Ube National College of Technology
Toshiharu YAKUSHI
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
Kazunobu MATSUSHITA
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

Keywords: acetic acid bacteria, Gluconacetobacter liquefaciens, 4-keto-D-arabonate, oxidative fermentation

JOURNAL FREE ACCESS

2010 Volume 74 Issue 12 Pages 2555-2558

DOI https://doi.org/10.1271/bbb.100698

View "Advance Publication" version (December 7, 2010).

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Abstract

Production of 4-keto-D-arabonate (4KAB) was confirmed in a culture medium of Gluconacetobacter liquefaciens strains, newly isolated from water kefir in Argentina. The strains rapidly oxidized D-glucose, D-gluconate (GA), and 2-keto-D-gluconate (2KGA), and accumulated 2,5-diketo-D-gluconate (25DKA) exclusively before reaching the stationary phase. 25DKA was in turn converted to 4KAB, and 4KAB remained stable in the culture medium. The occurrence of 4KAB was assumed by Ameyama and Kondo about 50 years ago in their study on the carbohydrate metabolism of acetic acid bacteria (Bull. Agr. Chem. Soc. Jpn., 22, 271–272, 380–386 (1958)). This is the first report confirming microbial production of 4KAB.

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