Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins

Shunsuke KOSAKA; Kazumasa HADA; Takashi NAKASHIMA; Makoto KIMURA

doi:10.1271/bbb.90577

Biochemistry & Molecular Biology Notes

Structural Changes in Ribonuclease P RNA in the Hyperthermophilic Archaeon Pyrococcus horikoshii OT3 Induced on Interaction with Proteins

Shunsuke KOSAKA, Kazumasa HADA, Takashi NAKASHIMA, Makoto KIMURA

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Keywords: hyperchronic effect, Pyrococcus horikoshii, protein-RNA interaction, RNA chaperone, RNase P

JOURNAL FREE ACCESS

2010 Volume 74 Issue 2 Pages 394-396

DOI https://doi.org/10.1271/bbb.90577

View "Advance Publication" version (February 7, 2010).

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Abstract

The activated structure of RNase P RNA (PhopRNA) in Pyrococcus horikoshii OT3 was characterized by circular dichroism (CD) and ultraviolet (UV) absorbance spectra. The results suggested that interaction of four RNase P proteins (PhoPop5, PhoRpp21, PhoRpp29, and PhoRpp30) with PhopRNA results in destabilization of base stacking in PhopRNA, whereas the addition of a fifth protein, PhoRpp38, increases base stacking in PhopRNA.

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