Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG; Xiaolan YANG; Juan FENG; Yonghua YUAN; Xiang LI; Youquan BU; Yanling XIE; Huidong YUAN; Fei LIAO

doi:10.1271/bbb.100080

Biochemistry & Molecular Biology Notes

Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG, Xiaolan YANG, Juan FENG, Yonghua YUAN, Xiang LI, Youquan BU, Yanling XIE, Huidong YUAN, Fei LIAO

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Keywords: hyperuricemia, uricase, PEG modification, thermo-stability

JOURNAL FREE ACCESS

2010 Volume 74 Issue 6 Pages 1298-1301

DOI https://doi.org/10.1271/bbb.100080

View "Advance Publication" version (June 7, 2010).

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Abstract

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

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