Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG; Xiaolan YANG; Juan FENG; Yonghua YUAN; Xiang LI; Youquan BU; Yanling XIE; Huidong YUAN; Fei LIAO

doi:10.1271/bbb.100080

Biochemistry & Molecular Biology Notes

Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG, Xiaolan YANG, Juan FENG, Yonghua YUAN, Xiang LI, Youquan BU, Yanling XIE, Huidong YUAN, Fei LIAO

Author information

Chun ZHANG
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Xiaolan YANG
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Juan FENG
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Yonghua YUAN
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Xiang LI
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Youquan BU
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Yanling XIE
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Huidong YUAN
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
Fei LIAO
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University

Keywords: hyperuricemia, uricase, PEG modification, thermo-stability

JOURNAL FREE ACCESS

2010 Volume 74 Issue 6 Pages 1298-1301

DOI https://doi.org/10.1271/bbb.100080

View "Advance Publication" version (June 7, 2010).

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Abstract

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

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