Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from Pseudomonas putida by Directed Evolution

Chie IMAMURA; Yasushi SHIGEMORI

doi:10.1271/bbb.100026

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from Pseudomonas putida by Directed Evolution

Chie IMAMURA, Yasushi SHIGEMORI

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Keywords: formaldehyde dehydrogenase, Pseudomonas putida, directed evolution, single-molecule PCR-linked in vitro expression, thermostability

JOURNAL FREE ACCESS

2010 Volume 74 Issue 7 Pages 1462-1465

DOI https://doi.org/10.1271/bbb.100026

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Abstract

We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 °C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 °C), the variants obtained retained <80% activity for at least 500 h (21 d).

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