The Contribution of Arabidopsis Homologs of <small>L</small>-Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid

Takanori MARUTA; Yaka ICHIKAWA; Takahiro MIEDA; Toru TAKEDA; Masahiro TAMOI; Yukinori YABUTA; Takahiro ISHIKAWA; Shigeru SHIGEOKA

doi:10.1271/bbb.100157

Biochemistry & Molecular Biology Notes

The Contribution of Arabidopsis Homologs of L-Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid

Takanori MARUTA, Yaka ICHIKAWA, Takahiro MIEDA, Toru TAKEDA, Masahiro TAMOI, Yukinori YABUTA, Takahiro ISHIKAWA, Shigeru SHIGEOKA

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Keywords: L-ascorbic acid, biosynthetic pathway, L-gulono-1,4-lactone oxidase, Arabidopsis plants

JOURNAL FREE ACCESS

2010 Volume 74 Issue 7 Pages 1494-1497

DOI https://doi.org/10.1271/bbb.100157

View "Advance Publication" version (July 7, 2010).

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Abstract

To clarify the involvement of seven Arabidopsis homologs of rat L-gulono-1,4-lactone (L-GulL) oxidase, AtGulLOs, in the biosynthesis of L-ascorbic acid (AsA), transgenic tobacco cells overexpressing the various AtGulLOs were generated. Under treatment with L-GulL, the levels of total AsA in three transgenic tobacco cell lines, overexpressing AtGulLO2, 3, or 5, were significantly increased as compared with those in control cells.

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