Turnover Ability of a Designed RNA Acting as a Template for Chemical Peptide Ligation

Kohei YAMASHITA; Norimasa KASHIWAGI; Hiroyuki FURUTA; Yoshiya IKAWA

doi:10.1271/bbb.110255

Biochemistry & Molecular Biology Notes

Turnover Ability of a Designed RNA Acting as a Template for Chemical Peptide Ligation

Kohei YAMASHITA, Norimasa KASHIWAGI, Hiroyuki FURUTA, Yoshiya IKAWA

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Keywords: RNA, native chemical ligation, Rev-peptide, N-peptide, P4–P6 RNA

JOURNAL FREE ACCESS

2011 Volume 75 Issue 10 Pages 2021-2024

DOI https://doi.org/10.1271/bbb.110255

View "Advance Publication" version (October 7, 2011).

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Abstract

A designed self-folding RNA possessing two peptide-recognition motifs served as a template for the chemical ligation of two RNA-binding peptides under stoichiometric conditions. In this study, we investigated the turnover ability of this template RNA in facilitation of peptide ligation and found that the RNA exhibited modest turnover ability under conditions in which its 3D structure was marginally stable.

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