Identification and Characterization of UDP-Glucose Dehydrogenase from the Hyperthermophilic Archaon, Pyrobaculum islandicum

Abstract

A gene encoding a UDP-glucose dehydrogenase homologue was identified in the hyperthermophilic archaeon, Pyrobaculum islandicum. This gene was expressed in Escherichia coli, and the product was purified and characterized. The expressed enzyme is the most thermostable UDP-glucose dehydrogenase so far described, with a half-life of 10 min at 90 °C. The enzyme retained its full activity after incubating in a pH range of 5.0–10.0 for 10 min at 80 °C. The temperature dependence of the kinetic parameters for this enzyme was examined at 37–70 °C. A decrease in K_ms for UDP-glucose and NAD was observed with decreasing temperature. This resulted in the enzyme still retaining high catalytic efficiency (V_max⁄K_m) for the substrate and cofactor, even at 37 °C. These characteristics make the enzyme potentially useful for its application at a much lower temperature such as 37 °C than the optimum growth temperature of 100 °C for P. islandicum.