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Bioscience, Biotechnology, and Biochemistry
Vol. 75 (2011) No. 11 P 2234-2236

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http://doi.org/10.1271/bbb.110246

Microbiology & Fermentation Technology Notes

Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.

Copyright © 2011 by Japan Society for Bioscience, Biotechnology, and Agrochemistry

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