1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa

Abstract

Using a superoxide (O₂⁻) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O₂⁻ generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O₂⁻ level in a dose-dependent manner with an IC₅₀ value of 21 μM, comparable to those of rosmarinic acid (RoA, IC₅₀=29 μM) and caffeic acid (CA, IC₅₀=30 μM). While RoA and CA also dose-dependently inhibited O₂⁻ generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O₂⁻ level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O₂⁻ scavengers.