Selective, High Conversion of <small>D</small>-Glucose to 5-Keto-<small>D</small>-gluoconate by Gluconobacter suboxydans

Yoshitaka ANO; Emiko SHINAGAWA; Osao ADACHI; Hirohide TOYAMA; Toshiharu YAKUSHI; Kazunobu MATSUSHITA

doi:10.1271/bbb.100701

Microbiology & Fermentation Technology Notes

Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans

Yoshitaka ANO, Emiko SHINAGAWA, Osao ADACHI, Hirohide TOYAMA, Toshiharu YAKUSHI, Kazunobu MATSUSHITA

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Keywords: acetic acid bacteria, Gluconobacter, oxidative fermentation, 5-keto-D-gluconate, quinoprotein glycerol dehydrogenase

JOURNAL FREE ACCESS

2011 Volume 75 Issue 3 Pages 586-589

DOI https://doi.org/10.1271/bbb.100701

View "Advance Publication" version (March 7, 2011).

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Abstract

Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5–4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity.

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