Cloning and Expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan SONG; Hiroyuki IMANAKA; Koreyoshi IMAMURA; Masashi MINODA; Toru KATASE; Yukiko HOSHI; Shotaro YAMAGUCHI; Kazuhiro NAKANISHI

doi:10.1271/bbb.110014

Biochemistry & Molecular Biology Notes

Cloning and Expression of a β-Galactosidase Gene of Bacillus circulans

Jingyuan SONG, Hiroyuki IMANAKA, Koreyoshi IMAMURA, Masashi MINODA, Toru KATASE, Yukiko HOSHI, Shotaro YAMAGUCHI, Kazuhiro NAKANISHI

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Keywords: β-galactosidase gene, Bacillus circulans, galactooligosaccharide

JOURNAL FREE ACCESS

2011 Volume 75 Issue 6 Pages 1194-1197

DOI https://doi.org/10.1271/bbb.110014

View "Advance Publication" version (June 13, 2011).

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Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta^®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

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