Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494

Takuo YAMAMOTO; Mio NISHIO-KOSAKA; Seisuke IZAWA; Hajime AGA; Tomoyuki NISHIMOTO; Hiroto CHAEN; Shigeharu FUKUDA

doi:10.1271/bbb.110116

Microbiology & Fermentation Technology Note

Enzymatic Properties of Recombinant Kojibiose Phosphorylase from Caldicellulosiruptor saccharolyticus ATCC43494

Takuo YAMAMOTO, Mio NISHIO-KOSAKA, Seisuke IZAWA, Hajime AGA, Tomoyuki NISHIMOTO, Hiroto CHAEN, Shigeharu FUKUDA

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Keywords: kojibiose, kojibiose phosphorylase, phosphorylase, glycoside hydrolase family 65, Caldicellulosiruptor saccharolyticus

JOURNAL FREE ACCESS

2011 Volume 75 Issue 6 Pages 1208-1210

DOI https://doi.org/10.1271/bbb.110116

View "Advance Publication" version (June 13, 2011).

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Abstract

One kojibiose phoshorylase (KP) homolog gene was cloned from Caldicellulosiruptor saccharolyticus ATCC43494. Recombinant KP from C. saccharolyticus (Cs-KP) expressed in Escherichia coli showed highest activity at pH 6.0 at 85 °C, and was stable from pH 3.5 to 10.0 and up to 85 °C for phosphorolysis. Cs-KP showed higher productivity of kojioligosaccharides of DP≧4 than KP from Thermoanaerobacter brockii ATCC35047.

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