A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans

Takahiro INOUE; Takashi YOSHIDA; Kei WADA; Takashi DAIFUKU; Keiichi FUKUYAMA; Yoshihiko SAKO

doi:10.1271/bbb.110159

Biochemistry & Molecular Biology Notes

A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans

Takahiro INOUE, Takashi YOSHIDA, Kei WADA, Takashi DAIFUKU, Keiichi FUKUYAMA, Yoshihiko SAKO

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Keywords: overexpression, metalloenzyme, CO dehydrogenase, CO₂ reduction

JOURNAL FREE ACCESS

2011 Volume 75 Issue 7 Pages 1392-1394

DOI https://doi.org/10.1271/bbb.110159

View "Advance Publication" version (July 7, 2011).

Biosci. Biotechnol. Biochem. : Vol. 75 (2011) No. 11 pp.2011E4

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Abstract

We established an Na₂S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO₂ reduction activity, of 16.9 U/mg.

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