Improvement of a Venus-Based Bimolecular Fluorescence Complementation Assay to Visualize bFos-bJun Interaction in Living Cells

Chika NAKAGAWA; Kazuto INAHATA; Shigenori NISHIMURA; Kenji SUGIMOTO

doi:10.1271/bbb.110189

Biochemistry & Molecular Biology Notes

Improvement of a Venus-Based Bimolecular Fluorescence Complementation Assay to Visualize bFos-bJun Interaction in Living Cells

Chika NAKAGAWA, Kazuto INAHATA, Shigenori NISHIMURA, Kenji SUGIMOTO

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Keywords: bimolecular fluorescence complementation (BiFC), fluorescent protein, protein interactions, Venus, signal-to-noise (S/N) ratio

JOURNAL FREE ACCESS

2011 Volume 75 Issue 7 Pages 1399-1401

DOI https://doi.org/10.1271/bbb.110189

View "Advance Publication" version (July 7, 2011).

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Abstract

Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction.

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