Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
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Occurrence, Biosynthesis, Biodegradation, and Industrial and Medical Applications of a Naturally Occurring ε-Poly-L-lysine
Yoshimitsu HAMANO
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2011 Volume 75 Issue 7 Pages 1226-1233


ε-Poly-L-lysine (ε-PL) consists of 25–35 L-lysine residues with linkages between the α-carboxyl groups and the ε-amino groups. It exhibits antimicrobial activity against a spectrum of microorganisms, including bacteria and fungi. Because of its high levels of safety and biodegradability, it is used as a food preservative in several countries. We recently identified an ε-PL synthetase (Pls) as a membrane protein, and investigated the catalytic mechanism. Pls was found to be an unusual non-ribosomal peptide synthetase (NRPS)-like peptide synthetase producing ε-PL with chain-length diversity. In addition, transcriptional analysis of pls and a kinetic study of Pls further suggested that the Pls catalytic function is regulated by intracellular ATP, high levels of which are required for full enzymatic activity. Furthermore, it was found that acidic pH conditions during ε-PL fermentation are necessary for the accumulation of intracellular ATP, rather than inhibition of the ε-PL-degrading enzyme.

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© 2011 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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