Production of Glycolipid Biosurfactants, Cellobiose Lipids, by Cryptococcus humicola JCM 1461 and Their Interfacial Properties

Abstract

Cryptococcus humicola JCM 1461 efficiently produced cellobiose lipids (CLs), bolaform biosurfactants. The main product was identified as 16-O-(2″,3″,4″,6′-tetra-O-acetyl-β-cellobiosyl)-2-hydroxyhexadecanoic acid. The production yield of CLs reached 13.1 g/L under the intermittent feeding of glucose. The critical micelle concentrations (CMC) of the main product at pH 4.0 and 7.0 were 3.3×10⁻⁵ M and 4.1×10⁻⁴ M respectively.