Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Note
Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol of Saccharomyces cerevisiae
Fumio MATSUDATakashi KONDOKengo IDAHironori TEZUKAJun ISHIIAkihiko KONDO
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JOURNALS FREE ACCESS

2012 Volume 76 Issue 11 Pages 2139-2141

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Abstract

To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L).

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© 2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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