Heterologous Expression of Corn Cystatin in Soybean and Effect on Growth of the Stink Bug

Masao ISHIMOTO; Masaharu KURODA; Koh-ichi YOZA; Keito NISHIZAWA; Masayoshi TERAISHI; Nobuo MIZUTANI; Kenji ITO; Seiichi MORIYA

doi:10.1271/bbb.120432

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Heterologous Expression of Corn Cystatin in Soybean and Effect on Growth of the Stink Bug

Masao ISHIMOTO, Masaharu KURODA, Koh-ichi YOZA, Keito NISHIZAWA, Masayoshi TERAISHI, Nobuo MIZUTANI, Kenji ITO, Seiichi MORIYA

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Keywords: cystatin, cysteine proteinase inhibitor, soybean, stink bug, transgenic

JOURNAL FREE ACCESS

2012 Volume 76 Issue 11 Pages 2142-2145

DOI https://doi.org/10.1271/bbb.120432

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Abstract

The bean bug (Riptortus clavatus) is a serious insect pest of soybean. Corn (maize) cystatin strongly inhibited the activity of its digestive cysteine proteinase. Heterologous expression of corn cystatin in soybean seeds inhibited the insect's proteases, but not its growth.

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