Enzymatic Assay for <small>D</small>-Aspartic Acid Using <small>D</small>-Aspartate Oxidase and Oxaloacetate Decarboxylase

Shiro KATO; Tomoyuki IKUTA; Hisashi HEMMI; Shoji TAKAHASHI; Yoshio KERA; Tohru YOSHIMURA

doi:10.1271/bbb.120477

Analytical Chemistry Note

Enzymatic Assay for D-Aspartic Acid Using D-Aspartate Oxidase and Oxaloacetate Decarboxylase

Shiro KATO, Tomoyuki IKUTA, Hisashi HEMMI, Shoji TAKAHASHI, Yoshio KERA, Tohru YOSHIMURA

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Keywords: D-amino acid, D-Asp assay, D-aspartate oxidase, α-keto acid, 1,2-diamino-4,5-methylenedioxybenzene (DMB)

JOURNAL FREE ACCESS

2012 Volume 76 Issue 11 Pages 2150-2152

DOI https://doi.org/10.1271/bbb.120477

View "Advance Publication" version (November 7, 2012).

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Abstract

An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino-4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.

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