Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation
Ahmed Magzoub KHALIDAtsushi ASANOYoshinao Z. HOSAKAMasanori OHTAKenji OHYAMAYoshiaki YAMANO
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JOURNAL FREE ACCESS

2012 Volume 76 Issue 11 Pages 2118-2123

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Abstract

We found that stem-cell leukemia (SCL), also known as T cell acute-lymphocytic leukemia (Tal-1) gene expression, was upregulated in the maturing rat testis. Strong expression of Tal-1 was detected in the normal maturing rat testis by Northern blotting. Western blotting revealed the protein size to be about 34 kDa. Protein expression was wide-spread in spermatocytes, spermtids and spermatogonia in accordance with the seminiferous epithelium cycle, as determined by an analysis of immunohistochemistry. Gene expression of Tal-1 regulatory gene, NKX3.1, was negatively correlated with Tal-1 expression. Human Tal-1 expression in the maturing testis as well as in bone marrow was observed, which suggests that the gene product is a novel cancer-testis antigen candidate. Taken together, TAL-1 may be involved in cell division, morphological changes, and the development of spermatogenic cells in the normal rat testis.

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© 2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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