Role of Lumenal Domain on Intracellular Localization of Tobacco Membrane-Anchored Prolyl 4-Hydroxylase

Abstract

NtP4H1.1 is a Golgi-localizing type II integral membrane protein. Mutations in the cytoplasmic tail direct the protein to the endoplasmic reticulum (ER). We expressed a GFP fusion protein containing the mutant tail and the transmembrane region in tobacco BY-2 cells, and found that the protein localized in the Golgi. Therefore NtP4H1.1 contains multiple targeting information in different regions.