Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science Regular Papers
Inhibitory Effects of Actinidiamide from Actinidia polygama on Allergy and Inflammation
Myun-Ho BANGIn-Gyeong CHAEEun-Ju LEENam-In BAEKYoon-Su BAEKDae-Young LEEIn-Seon LEESam-Pin LEESeun-Ah YANG
Author information

2012 Volume 76 Issue 2 Pages 289-293


Actinidia polygama Max. was subjected to supercritical fluid extraction (SFE), and the resulting ethanol extract of marc (SFEM) was subjected to sequential fractionation with various solvents. Each extract and fraction was assayed for anti-inflammatory effect. The ethyl acetate fraction (EtOAc) contained the highest level (70.8% inhibition) of anti-inflammatory activity. In order to identify the active constituents, the EtOAc fraction was further fractionated by silica gel and ODS column chromatography. By activity-guided fractionation, an active ceramide was identified as the anti-inflammatory component, and its structure was determined by NMR and MS analysis. The novel ceramide was named actinidiamide, and was found significantly to inhibit nitric oxide (NO) production (30.6% inhibition at 1 μg/mL) in lipopolysaccaride (LPS)-stimulated RAW 264.7 cells and β-hexosaminidase release (91.8% inhibition at 1 μg/mL) in IgE-sensitized RBL-2H3 cells. Thus the presence of actinidiamide conveys allergy and inflammation treatment ability to A. polygama.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article