Separation and Identification of Rice Prolamins by Two-Dimensional Gel Electrophoresis and Amino Acid Sequencing

Takanari SHIGEMITSU; Yuhi SAITO; Shigeto MORITA; Shigeru SATOH; Takehiro MASUMURA

doi:10.1271/bbb.110791

Biochemistry & Molecular Biology Notes

Separation and Identification of Rice Prolamins by Two-Dimensional Gel Electrophoresis and Amino Acid Sequencing

Takanari SHIGEMITSU, Yuhi SAITO, Shigeto MORITA, Shigeru SATOH, Takehiro MASUMURA

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Keywords: rice seed, prolamin, 2D-PAGE, amino acid sequencing

JOURNAL FREE ACCESS

2012 Volume 76 Issue 3 Pages 594-597

DOI https://doi.org/10.1271/bbb.110791

View "Advance Publication" version (March 7, 2012).

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Abstract

There are difficulties in detecting and separating rice prolamin polypeptides by 2D-PAGE analysis because prolamin polypeptides are insoluble, and the amino acid sequences show high homology among them. In this study, we improved the prolamin extraction method and the 2D-PAGE procedure, and succeeded in separating prolamin polypeptide species by 2D-PAGE and in identifying major prolamin polypeptide sequences.

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