AxyR, an AraC Family Transcriptional Activator of the Xylanase 3 Gene, Requires Xylo-Oligosaccharides as a Cofactor for DNA Binding in Paenibacillus sp. Strain W-61

Mutsumi FUKUDA; Shigeki YOSHIDA; Hiroya ITOH; Yoji KATO; Seiji WATANABE; Yoshifumi ITOH; Yoshiyuki KAMIO; Jun KANEKO

doi:10.1271/bbb.120165

Microbiology & Fermentation Technology Communication

AxyR, an AraC Family Transcriptional Activator of the Xylanase 3 Gene, Requires Xylo-Oligosaccharides as a Cofactor for DNA Binding in Paenibacillus sp. Strain W-61

Mutsumi FUKUDA, Shigeki YOSHIDA, Hiroya ITOH, Yoji KATO, Seiji WATANABE, Yoshifumi ITOH, Yoshiyuki KAMIO, Jun KANEKO

著者情報

キーワード: xylanase, Paenibacillus, co-factor, xylo-oligosaccharides, DNA-binding protein

ジャーナルフリー

2012 年 76 巻 5 号 p. 1051-1054

DOI https://doi.org/10.1271/bbb.120165

View "Advance Publication" version (May 7, 2012).

詳細

抄録

The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

責任著者(Corresponding author)

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