Establishment of a Monitoring System to Detect Inhibition of mRNA Processing

Ken-ichi FUJITA; Masumi OKAMURA; Sachiko NISHIMOTO; Tomoya KURIHARA; Keiko MOMMA; Yusaku MIYAMAE; Taiho KAMBE; Masaya NAGAO; Hiroshi NARITA; Seiji MASUDA

doi:10.1271/bbb.120226

Biochemistry & Molecular Biology Communications

Establishment of a Monitoring System to Detect Inhibition of mRNA Processing

Ken-ichi FUJITA, Masumi OKAMURA, Sachiko NISHIMOTO, Tomoya KURIHARA, Keiko MOMMA, Yusaku MIYAMAE, Taiho KAMBE, Masaya NAGAO, Hiroshi NARITA, Seiji MASUDA

Author information

Ken-ichi FUJITA
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Masumi OKAMURA
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Sachiko NISHIMOTO
Department of Food and Nutrition, Kyoto Women's University
Tomoya KURIHARA
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Keiko MOMMA
Department of Living and Welfare, Kyoto Women's University
Yusaku MIYAMAE
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Taiho KAMBE
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Masaya NAGAO
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Hiroshi NARITA
Department of Food and Nutrition, Kyoto Women's University
Seiji MASUDA
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

Keywords: mRNA processing, inhibitor, luciferase reporter, RNA-fluorescence in situ hybridyzation (FISH), reverse transcription-polymerase chain reaction (RT-PCR)

JOURNAL FREE ACCESS

2012 Volume 76 Issue 6 Pages 1248-1251

DOI https://doi.org/10.1271/bbb.120226

View "Advance Publication" version (June 7, 2012).

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Abstract

A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

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