Establishment of a Monitoring System to Detect Inhibition of mRNA Processing

Ken-ichi FUJITA; Masumi OKAMURA; Sachiko NISHIMOTO; Tomoya KURIHARA; Keiko MOMMA; Yusaku MIYAMAE; Taiho KAMBE; Masaya NAGAO; Hiroshi NARITA; Seiji MASUDA

doi:10.1271/bbb.120226

Biochemistry & Molecular Biology Communications

Establishment of a Monitoring System to Detect Inhibition of mRNA Processing

Ken-ichi FUJITA, Masumi OKAMURA, Sachiko NISHIMOTO, Tomoya KURIHARA, Keiko MOMMA, Yusaku MIYAMAE, Taiho KAMBE, Masaya NAGAO, Hiroshi NARITA, Seiji MASUDA

著者情報

キーワード: mRNA processing, inhibitor, luciferase reporter, RNA-fluorescence in situ hybridyzation (FISH), reverse transcription-polymerase chain reaction (RT-PCR)

ジャーナルフリー

2012 年 76 巻 6 号 p. 1248-1251

DOI https://doi.org/10.1271/bbb.120226

View "Advance Publication" version (June 7, 2012).

詳細

抄録

A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.

責任著者(Corresponding author)

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