Extraction of High-Quality RNA from Rubber Tree Leaves

Liu-Hong DENG; Ming-Wu LUO; Chun-Fa ZHANG; Hui-Cai ZENG

doi:10.1271/bbb.120014

Biochemistry & Molecular Biology Notes

Extraction of High-Quality RNA from Rubber Tree Leaves

Liu-Hong DENG, Ming-Wu LUO, Chun-Fa ZHANG, Hui-Cai ZENG

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Keywords: rubber-tree leaves, RNA extraction, cetyltrimethylammonium bromide (CTAB), TRIzol, rapid amplification of cDNA ends (RACE)

JOURNAL FREE ACCESS

2012 Volume 76 Issue 7 Pages 1394-1396

DOI https://doi.org/10.1271/bbb.120014

View "Advance Publication" version (July 7, 2012).

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Abstract

A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.

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