Vitamin D Receptor Is Not Essential for Extracellular Signal-Related Kinase Phosphorylation by Vitamin D3 in Human Caco-2/TC7 Cells

Jun YAMAUCHI; Mariko SEKIGUCHI; Tomomi SHIRAI; Yoshiko ISHIMI

doi:10.1271/bbb.120298

Biochemistry & Molecular Biology Notes

Vitamin D Receptor Is Not Essential for Extracellular Signal-Related Kinase Phosphorylation by Vitamin D₃ in Human Caco-2/TC7 Cells

Jun YAMAUCHI, Mariko SEKIGUCHI, Tomomi SHIRAI, Yoshiko ISHIMI

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Keywords: vitamin D, nongenomic action, vitamin D receptor (VDR), extracellular signal-related kinase (ERK), Caco-2/TC7 cell

JOURNAL FREE ACCESS

2012 Volume 76 Issue 8 Pages 1588-1590

DOI https://doi.org/10.1271/bbb.120298

View "Advance Publication" version (August 7, 2012).

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Abstract

Vitamin D₃ initiated rapid extracellular signal-related kinase (ERK) phosphorylation, but the contribution of vitamin D receptor (VDR) to this event is unclear. We investigated the use of RNA interference (RNAi) to knockdown VDR. RNAi downregulated VDR as well as its targeted gene expression, but vitamin D₃ dependent ERK phosphorylation remained. Thus VDR might not be involved in ERK phosphorylation by vitamin D₃.

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