Isolation and Characterization of an Anti-Insect β-Toxin from the Venom of the Scorpion Isometrus maculatus

Tomoyuki KAWACHI; Masahiro MIYASHITA; Yoshiaki NAKAGAWA; Hisashi MIYAGAWA

doi:10.1271/bbb.120697

Organic Chemistry Note

Isolation and Characterization of an Anti-Insect β-Toxin from the Venom of the Scorpion Isometrus maculatus

Tomoyuki KAWACHI, Masahiro MIYASHITA, Yoshiaki NAKAGAWA, Hisashi MIYAGAWA

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Keywords: scorpion β-toxin, insect toxicity, HPLC, de novo sequencing, Edman degradation

JOURNAL FREE ACCESS

2013 Volume 77 Issue 1 Pages 205-207

DOI https://doi.org/10.1271/bbb.120697

View "Advance Publication" version (January 7, 2013).

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Abstract

Im-3 was isolated from the venom of the scorpion Isometrus maculatus through several steps of HPLC fractionation based on the insect paralytic activity. Injecting Im-3 into crickets induced paralysis, but no toxicity was apparent in mice after an intracerebroventricular injection. Im-3 shares sequence similarity to scorpion β-toxins that specifically affect insect sodium channels.

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