Characterization of Polyphosphate Glucokinase SCO5059 from Streptomyces coelicolor A3(2)

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SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The K_m values of SCO5059 for glucose and polyphosphate (poly(P)₆) were estimated to be 12 and 4 µM, respectively, and the k_cat value was 0.3 s⁻¹ at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.