Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts

Yuki HAMAMOTO; Takuji TSUKIYAMA; Yoshihiro YOSHIKAKE; Masayoshi TERAISHI; Yutaka OKUMOTO; Takatoshi TANISAKA

doi:10.1271/bbb.130480

Biochemistry & Molecular Biology Notes

Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts

Yuki HAMAMOTO, Takuji TSUKIYAMA, Yoshihiro YOSHIKAKE, Masayoshi TERAISHI, Yutaka OKUMOTO, Takatoshi TANISAKA

Author information

Keywords: rice, protoplast, transient reporter assay, translation efficiency, GVG system

JOURNAL FREE ACCESS

2013 Volume 77 Issue 12 Pages 2480-2482

DOI https://doi.org/10.1271/bbb.130480

View "Advance Publication" version (December 7, 2013).

Details

Abstract

We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.

Corresponding author

Register with J-STAGE for free!