Measurement of Barley β-Glucan Concentration in the Plasma by Sandwich ELISA Using Rat Dectin-1

Abstract

We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barley β-glucan (BβG). The ELISA method had a working range of 15–4,000 µg/L. Plasma BβG was detectable up to 24 h after an intravenous administration of BβG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BβG.