Bio-Nanocapsules for Signal Enhancement of Alkaline Phosphatase-Linked Immunosorbent Assays

Masumi IIJIMA; Mikako YAMAMOTO; Nobuo YOSHIMOTO; Tomoaki NIIMI; Shun'ichi KURODA

doi:10.1271/bbb.120760

Analytical Chemistry Notes

Bio-Nanocapsules for Signal Enhancement of Alkaline Phosphatase-Linked Immunosorbent Assays

Masumi IIJIMA, Mikako YAMAMOTO, Nobuo YOSHIMOTO, Tomoaki NIIMI, Shun'ichi KURODA

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Keywords: bio-nanocapsules, alkaline phosphatase, horseradish peroxidase, oriented immobilization, cluster formation

JOURNAL FREE ACCESS

2013 Volume 77 Issue 4 Pages 843-846

DOI https://doi.org/10.1271/bbb.120760

View "Advance Publication" version (April 7, 2013).

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Abstract

The bio-nanocapsules displaying about 240 molecules of immunoglobulin G Fc-binding Z domains (ZZ-BNCs) enhanced the signals of enzyme-linked immunosorbent assay by tethering the Fc regions of secondary antibodies (Abs), which were eliminated using high-molecular mass enzymes (e.g., alkaline phosphatase). By way of optimizing the distance between enzymes and Abs, ZZ-BNCs improved sensitivity independently of enzymes.

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